Description: Rabbit polyclonal antibody to RPS25 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RPS25. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RPS25 Alternative Names: 40S ribosomal protein S25Entrez Gene (Human): 6230Entrez Gene (Mouse) : 75617Entrez Gene (Rat) : 122799SwissProt (Human): P62851SwissProt (Mouse) : P62852SwissProt (Rat) : P62853Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPS25 expression in HEK293T (A), Myla2059 (B), HUT78 (C), mouse liver (D), rat liver (E) whole cell lysates.
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Immunohistochemical analysis of RPS25 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.