Description: Rabbit polyclonal antibody to BRSK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRSK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: BRSK1 Alternative Names: KIAA1811; SAD1; SADB; Serine/threonine-protein kinase BRSK1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1; SAD1 homolog; hSAD1Entrez Gene (Human): 84446Entrez Gene (Mouse) : 381979SwissProt (Human): Q8TDC3SwissProt (Mouse) : Q5RJI5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BRSK1 expression in Hela (A), CT26 (B) whole cell lysates.
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Immunofluorescent analysis of BRSK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.