Description: Rabbit polyclonal antibody to FOXO4 (Phospho-S262) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S262 of human FOXO4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FOXO4 Alternative Names: AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1Entrez Gene (Human): 4303Entrez Gene (Mouse) : 54601SwissProt (Human): P98177SwissProt (Mouse) : Q9WVH3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FOXO4 (Phospho-S262) expression in mouse muscle (A), rat brain (B), rat muscle (C) whole cell lysates.
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Immunohistochemical analysis of FOXO4 (Phospho-S262) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.