Description: Rabbit polyclonal antibody to OCT1 (Phospho-S385) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S385 of human 45200 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: POU2F1 Alternative Names: POU2F1; OCT1; OTF1; POU domain class 2 transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1; POU2F2; OCT2; OTF2; POU domain class 2 transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2Entrez Gene (Human): 5451; 5452Entrez Gene (Mouse) : 18986; 18987Entrez Gene (Rat) : 171068SwissProt (Human): P14859; P09086SwissProt (Mouse) : P25425; Q00196SwissProt (Rat) : P31503Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of OCT1 (Phospho-S385) expression in mouse lung (A), rat lung (B) whole cell lysates.
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Immunohistochemical analysis of OCT1 (Phospho-S385) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.