Description: Rabbit polyclonal antibody to CD247 (Phospho-Y142) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y142 of human CD247 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CD247 Alternative Names: CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247Entrez Gene (Human): 919Entrez Gene (Mouse) : 12503SwissProt (Human): P20963SwissProt (Mouse) : P24161Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD247 (Phospho-Y142) expression in HEK293T (A), LOVO (B), HCT116 (C), mouse liver (D) whole cell lysates.
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  Immunohistochemical analysis of CD247 (Phospho-Y142) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-CD247 (Phospho-Y142) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.