Description: Rabbit polyclonal antibody to CD294 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD294. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PTGDR2 Alternative Names: CRTH2; DL1R; GPR44; Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD294Entrez Gene (Human): 11251Entrez Gene (Rat) : 309212SwissProt (Human): Q9Y5Y4SwissProt (Rat) : Q6XKD3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates.
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  Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.