Description: Rabbit polyclonal antibody to MUC2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MUC2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: MUC2 Alternative Names: SMUC; Mucin-2; MUC-2; Intestinal mucin-2Entrez Gene (Human): 4583Entrez Gene (Mouse) : 17831Entrez Gene (Rat) : 24572SwissProt (Human): Q02817SwissProt (Mouse) : Q80Z19SwissProt (Rat) : Q62635Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of MUC2 expression in mouse small intestine (A), rat small intestine (B) whole cell lysates.
-
Immunohistochemical analysis of MUC2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Immunofluorescent analysis of MUC2 staining in mouse colon. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).