Description: Rabbit polyclonal antibody to PDGFR alpha (Phospho-Y754) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y754 of human PDGFR alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PDGFRA Alternative Names: PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD140aEntrez Gene (Human): 5156Entrez Gene (Mouse) : 18595Entrez Gene (Rat) : 25267SwissProt (Human): P16234SwissProt (Mouse) : P26618SwissProt (Rat) : P20786Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PDGFR alpha (Phospho-Y754) expression in Hela (A) whole cell lysates.
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Immunohistochemical analysis of PDGFR alpha (Phospho-Y754) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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