Description: Rabbit polyclonal antibody to NOD2 Immunogen: Recombinant protein corresponding to human NOD2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NOD2 Alternative Names: CARD15; IBD1; Nucleotide-binding oligomerization domain-containing protein 2; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1Entrez Gene (Human): 64127SwissProt (Human): Q9HC29SwissProt (Mouse) : Q8K3Z0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NOD2 expression in mouse kidney (A), rat spleen (B) whole cell lysates.
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Immunohistochemical analysis of NOD2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.