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Rapid Transfer Buffer (10X) (Powder) CRG1040
Product NameRapid Transfer Buffer (10X) (Powder)
Cat No: CRG1040
Source:
Reactivity:
Applications: WB
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1L
$20
100 ml
$5
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Description: Quick, efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
Directions for Use : Stock Solution: 1L size: Dissolve the powder in 1L deionized water for Rapid Transfer Buffer (10X). 100 ml size: Dissolve the powder in 100 ml deionized water for Rapid Transfer Buffer (10X). Working Solution: Prepare 1 L of Rapid Transfer Buffer (1X) by diluting 100 mL of Rapid Transfer Buffer (10X) with 800 mL of deionized water, then add 100 ml Ethanol.
Application : Wet Transfer Protocol 1. Prepare membrane and filter paper for transfer: a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel. Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer. b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes. 2. Following protein electrophoresis, assemble the blotting sandwich following the manufacturer’s instructions for the transfer apparatus. 3. Place the blotting sandwich in a wet transfer tank filled with 1X Rapid Transfer Buffer. 4. Transfer constant current 300 - 400 mA at room temperature. Semi-Dry Transfer Protocol 1. Prepare membrane and filter paper for transfer: a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel. Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer. b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes. 2. Following electrophoresis, wash the gel for 2 minutes in deionized water. 3. Pre-equilibrate the gel in 1X Rapid Transfer Buffer for 5 minutes. 4. Assemble the blotting sandwich following the manufacturer’s instructions for the semi-dry transfer apparatus. 5. Transfer constant current 300 - 400 mA at room temperature.
Storage/Stability : Store at 25°C for 2 years.
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