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Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.

Product Name:	Mouse Anti-Rabbit IgG - HRP For IP/CoIP
Cat No:	CSA9108
Source:	Mouse
Reactivity:	Rb
Applications:	IP, CoIP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

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100 μl

500 μl

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Datasheet

MSDS

Description：Mouse Monoclonal Secondary Antibody to Rabbit IgG HRP labeled

Immunogen：Rabbit IgG

Purification：The antibody is isolated from cell culture by protein A/G immunoaffinity chromatography.

Clonality：Monoclonal

Conjugation：HRP

Form：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% Proclin

Dilution：WB (1/500 - 1/2000)

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.

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