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CRP2817
Product Name:Recombinant Human SMAD2 Protein
Cat No:CRP2817
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
10 μg
130
50 μg
390
500 μg
1950
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Description:Recombinant Human SMAD2 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser467 is expressed with a 6His, Flag tag at the N-terminus.
Form:Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH7.5.
Gene Symbol:SMAD2
Alternative Names:MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Entrez Gene (Human): 4087;
SwissProt (Human): Q15796;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MRGSHHHHHH GSDYKDDDDK SSILPFTPPV VKRLLGWKKS AGGSGGAGGG EQNGQEEKWC EKAVKSLVKK LKKTGRLDEL EKAITTQNCN TKCVTIPSTC SEIWGLSTPN TIDQWDTTGL YSFSEQTRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELKAIENCEY AFNLKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEILTELP PLDDYTHSIP ENTNFPAGIE PQSNYIPETP PPGYISEDGE TSDQQLNQSM DTGSPAELSP TTLSPVNHSL DLQPVTYSEP AFWCSIAYYE LNQRVGETFH ASQPSLTVDG FTDPSNSERF CLGLLSNVNR NATVEMTRRH IGRGVRLYYI GGEVFAECLS DSAIFVQSPN CNQRYGWHPA TVCKIPPGCN LKIFNNQEFA ALLAQSVNQG FEAVYQLTRM CTIRMSFVKG WGAEYRRQTV TSTPCWIELH LNGPLQWLDK VLTQMGSPSV RCSSMS
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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