Description:Rabbit polyclonal antibody to ALDH7A1Immunogen:Recombinant full length protein of human ALDH7A1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:ALDH7A1Alternative Names:ATQ1; Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase
Entrez Gene (Human):
501;
Entrez Gene (Mouse):
110695;
Entrez Gene (Rat):
291450;
SwissProt (Human):
P49419;
SwissProt (Mouse):
Q9DBF1;
SwissProt (Rat):
Q64057;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ALDH7A1 expression in SKOV3 (A), Hela (B), mouse kidney (C), rat liver (D) whole cell lysates. (Predicted band size: 51; 55; 58 kD; Observed band size: 58 kD)
-
Immunofluorescent analysis of ALDH7A1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).