Description:Recombinant E. coli MDH Protein is produced by E. coli expression system and the target gene encoding Met1-Lys312 is expressed with a 6His tag at the N-terminus.Form:Supplied as a 0.2 μM filtered solution of 50mM PB, 50% Glycerol, pH 7.5.Gene Symbol:MDHAlternative Names:Malate dehydrogenase
Entrez Gene (Human):
947854 (E. coli);
SwissProt (Human):
P61889 (E. coli);
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGFSG EDATPALEGA DVVLISAGVR RKPGMDRSDL FNVNAGIVKN LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLLGVTTLD IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK KDIALGQEFV NKDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.