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CMA4410
  • Western blot analysis of TMOD2 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 39; 35 kD; Observed band size: 40 kD)
  • Immunofluorescent analysis of TMOD2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Recombinant Anti-TMOD2 Rabbit mAb
Cat No:CMA4410
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Recombinant rabbit monoclonal antibody to TMOD2
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human TMOD2. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Monoclonal
Form:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol:TMOD2
Alternative Names:NTMOD; Tropomodulin-2; Neuronal tropomodulin; N-Tmod
Entrez Gene (Human): 29767;
Entrez Gene (Mouse): 50876;
Entrez Gene (Rat): 58814;
SwissProt (Human): Q9NZR1;
SwissProt (Mouse): Q9JKK7;
SwissProt (Rat): P70566;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of TMOD2 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 39; 35 kD; Observed band size: 40 kD)
  • Immunofluorescent analysis of TMOD2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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