Description:Recombinant Human CPE Protein is produced by mammalian expression system and the target gene encoding Arg42-Ser453 is expressed with a 6His tag at the C-terminus.Form:Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.5.Gene Symbol:CPEAlternative Names:Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase
Entrez Gene (Human):
1363;
SwissProt (Human):
P16870;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:RLQQEDGISF EYHRYPELRE ALVSVWLQCT AISRIYTVGR SFEGRELLVI ELSDNPGVHE PGEPEFKYIG NMHGNEAVGR ELLIFLAQYL CNEYQKGNET IVNLIHSTRI HIMPSLNPDG FEKAASQPGE LKDWFVGRSN AQGIDLNRNF PDLDRIVYVN EKEGGPNNHL LKNMKKIVDQ NTKLAPETKA VIHWIMDIPF VLSANLHGGD LVANYPYDET RSGSAHEYSS SPDDAIFQSL ARAYSSFNPA MSDPNRPPCR KNDDDSSFVD GTTNGGAWYS VPGGMQDFNY LSSNCFEITV ELSCEKFPPE ETLKTYWEDN KNSLISYLEQ IHRGVKGFVR DLQGNPIANA TISVEGIDHD VTSAKDGDYW RLLIPGNYKL TASAPGYLAI TKKVAVPYSP AAGVDFELES FSVDHHHHHHDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.