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CRP1272
Product Name:Recombinant Human CDK4 Protein
Cat No:CRP1272
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
10 μg
140
50 μg
420
500 μg
2520
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Description:Recombinant Human CDK4 Protein is produced by E. coli expression system and the target gene encoding Met1-Glu303 is expressed with a 6His, T7 tag at the N-terminus.
Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Gene Symbol:CDK4
Alternative Names:Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3
Entrez Gene (Human): 1019;
SwissProt (Human): P11802;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMATSRY EPVAEIGVGA YGTVYKARDP HSGHFVALKS VRVPNGGGGG GGLPISTVRE VALLRRLEAF EHPNVVRLMD VCATSRTDRE IKVTLVFEHV DQDLRTYLDK APPPGLPAET IKDLMRQFLR GLDFLHANCI VHRDLKPENI LVTSGGTVKL ADFGLARIYS YQMALTPVVV TLWYRAPEVL LQSTYATPVD MWSVGCIFAE MFRRKPLFCG NSEADQLGKI FDLIGLPPED DWPRDVSLPR GAFPPRGPRP VQSVVPEMEE SGAQLLLEML TFNPHKRISA FRALQHSYLH KDEGNPE
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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