Description:Recombinant Human BDNF Protein is produced by E. coli. It is a single non-glycosylated polypeptide chain containing 119 amino acids. Bioactive BDNF is a non-covalently linked homodimer with a molecular mass of 27 kD.Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2.Gene Symbol:BDNFAlternative Names:Brain-derived neurotrophic factor; BDNF; Abrineurin
Entrez Gene (Human):
627;
SwissProt (Human):
P23560;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure:MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGRDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.