Description:Recombinant Human BCMO1 Protein is produced by mammalian expression system and the target gene encoding Met1-Thr547 is expressed with a 6His tag at the C-terminus.Form:Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 10mM reduced Glutathione, pH 8.0.Gene Symbol:BCMO1Alternative Names:BCDO; BCDO1; Betabeta-carotene 1515'-monooxygenase; Beta-carotene dioxygenase 1
Entrez Gene (Human):
53630;
SwissProt (Human):
Q9HAY6;
Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MDIIFGRNRK EQLEPVRAKV TGKIPAWLQG TLLRNGPGMH TVGESRYNHW FDGLALLHSF TIRDGEVYYR SKYLRSDTYN TNIEANRIVV SEFGTMAYPD PCKNIFSKAF SYLSHTIPDF TDNCLINIMK CGEDFYATSE TNYIRKINPQ TLETLEKVDY RKYVAVNLAT SHPHYDEAGN VLNMGTSIVE KGKTKYVIFK IPATVPEGKK QGKSPWKHTE VFCSIPSRSL LSPSYYHSFG VTENYVIFLE QPFRLDILKM ATAYIRSMSW ASCLAFHREE KTYIHIIDQR TRQPVQTKFY TDAMVVFHHV NAYEEDGCIV FDVIAYEDNS LYQLFYLANL NQDFKENSRL TSVPTLRRFA VPLHVDKNAE VGTNLIKVAS TTATALKEED GQVYCQPEFL YEGLELPRVN YAHNGKQYRY VFATGVQWSP IPTKIIKYDI LTKSSLKWRE DDCWPAEPLF VPAPGAKDED DGVILSAIVS TDPQKLPFLL ILDAKSFTEL ARASVDVDMH MDLHGLFITD MDWDTKKQAA SEEQRDRASD CHGAPLTVDH HHHHHDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.