Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CQA1879
  • Western blot analysis of CD36 expression in mouse heart (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 100; 53 kD)
  • Immunofluorescent analysis of CD36 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-CD36 Antibody
Cat No:CQA1879
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to CD36
Immunogen:Recombinant full length protein of human CD36
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol:CD36
Alternative Names:GP3B; GP4; Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD36
Entrez Gene (Human): 948;
Entrez Gene (Mouse): 12491;
SwissProt (Human): P16671;
SwissProt (Mouse): Q08857;
SwissProt (Rat): Q07969;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CD36 expression in mouse heart (A), mouse muscle (B), rat heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 100; 53 kD)
  • Immunofluorescent analysis of CD36 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
RFX1 regulates foam cell formation and atherosclerosis by mediating CD36 expression
Journal Int Immunopharmacol
IF 5.6
Application WB
Reactivity Mouse
PMID 38402833
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved