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CRP1082
Product Name:Recombinant Human ASF1A Protein
Cat No:CRP1082
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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Description:Recombinant Human ASF1A Protein is produced by E. coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
Form:Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.
Gene Symbol:ASF1A
Alternative Names:Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA
Entrez Gene (Human): 25842;
SwissProt (Human): Q9Y294;
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure:MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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