Description:Rabbit polyclonal antibody to DHRS9Immunogen:Recombinant full length protein of human DHRS9Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:DHRS9Alternative Names:Dehydrogenase/reductase SDR family member 9; 3-alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; RDHL; Short-chain dehydrogenase/reductase retSDR8; Tracheobronchial epithelial cell-specific retinol dehydrogenase; RDH-TBE
Entrez Gene (Human):
10170;
Entrez Gene (Mouse):
241452;
Entrez Gene (Rat):
170635;
SwissProt (Human):
Q9BPW9;
SwissProt (Mouse):
Q58NB6;
SwissProt (Rat):
Q8VD48;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DHRS9 expression in HT29 (A), THP1 (B), mouse pancreas (C), rat trachea (D) whole cell lysates. (Predicted band size: 18; 30; 35; 41 kD; Observed band size: 35 kD)
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Immunofluorescent analysis of DHRS9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.