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CRP1063
Product Name:Recombinant Human APEX1 Protein
Cat No:CRP1063
Source:E. coli
Reactivity:H
Applications:E, WB, SDS-PAGE, MS
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
10 μg
140
50 μg
420
500 μg
2100
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Description:Recombinant Human APEX1 Protein is produced by E.coli expression system and the target gene encoding Pro2-Leu318 is expressed.
Form:Liquid in a 0.2 μM filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.
Gene Symbol:APEX1
Alternative Names:APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1
Entrez Gene (Human): 328;
SwissProt (Human): P27695;
Purity:Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Chemical Structure:MGPKRGKKGA VAEDGDELRT EPEAKKSKTA AKKNDKEAAG EGPALYEDPP DQKTSPSGKP ATLKICSWNV DGLRAWIKKK GLDWVKEEAP DILCLQETKC SENKLPAELQ ELPGLSHQYW SAPSDKEGYS GVGLLSRQCP LKVSYGIGEE EHDQEGRVIV AEFDSFVLVT AYVPNAGRGL VRLEYRQRWD EAFRKFLKGL ASRKPLVLCG DLNVAHEEID LRNPKGNKKN AGFTPQERQG FGELLQAVPL ADSFRHLYPN TPYAYTFWTY MMNARSKNVG WRLDYFLLSH SLLPALCDSK IRSKALGSDH CPITLYLAL
Directions for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability:Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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