Description:ELISA Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection.Form:Liquid
Directions for Use:(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20.
(b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved.
(c) Add 100 μl of ELISA Stop Solution.
(d) Read at Absorbance 450 nm.Storage/Stability:Store at 4°C for one year.