Description：ECL Detection Reagent (Femtogram level) is designed for the detection low femtogram level proteins on all routine Western blottingForm：Liquid Directions for Use：Mix ECL Detection Reagent A and B at 1:1. Use 0.1 ml mix solution per cm2 of membrane. For best results prepare mix solution immediately before use. The mix solution is stable for up to 3 hour at room temperature in dark.Components：ECL Detection Reagent A, ECL Detection Reagent BApplication：ECL Detection Reagent uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagent results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.Storage/Stability：Store at 4 °C for one year. Do not freeze.