Description：AcalephFluor®647 Conjugation Kit uses a simple and quick process for AcalephFluor®647 labeling/conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Directions for Use：1. Reaction Buffer, DMSO, Quencher reagent All reagents must be warmed to the room temperature before use. 2. AcalephFluor®647 For label 1 mg protein size: Add 10 μl DMSO into the AcalephFluor®647 tube. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette. The operation should be at room temperature, DMSO will turn to a solid at lower temperature. The dissolved reagent can be stored at -20°C for 2 weeks. For label 5 mg protein size: Add 50 μl DMSO into the AcalephFluor®647 tube. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette. The operation should be at room temperature, DMSO will turn to a solid at lower temperature. The dissolved reagent can be stored at -20°C for 2 weeks. 3. Antibody The concentration of the antibody which need to be labeled, should be higher than 1 mg/ml. The lower concentration will affect conjugation efficiency.Storage/Stability：Shipped at 4°C. Store at -20°C and protect from light for 2 years.Platform：Ex/Em = 650/665 nm