Description:Rabbit polyclonal antibody to CCAR1Immunogen:Recombinant full length protein of human CCAR1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)Gene Symbol:CCAR1Alternative Names:CARP1; DIS; Cell division cycle and apoptosis regulator protein 1; Cell cycle and apoptosis regulatory protein 1; CARP-1; Death inducer with SAP domain
Entrez Gene (Human):
55749;
Entrez Gene (Mouse):
67500;
SwissProt (Human):
Q8IX12;
SwissProt (Mouse):
Q8CH18;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCAR1 expression in Jurkat (A), HepG2 (B), A549 (C), A431 (D) whole cell lysates. (Predicted band size: 132 kD; Observed band size: 155 kD)
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Immunofluorescent analysis of CCAR1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.