Description：Rabbit polyclonal antibody to ATG7Immunogen：Recombinant full length protein of human ATG7Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：ATG7Alternative Names：APG7L; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein Entrez Gene (Human)： 10533; Entrez Gene (Mouse)： 74244; Entrez Gene (Rat)： 312647; SwissProt (Human)： O95352; SwissProt (Mouse)： Q9D906; SwissProt (Rat)： Q641Y5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATG7 expression in THP1 (A), HeLa (B), mouse kidney (C), rat liver (D) whole cell lysates. (Predicted band size: 68; 75; 77 kD; Observed band size: 78 kD)
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  Immunohistochemical analysis of ATG7 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of ATG7 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).