Description:Rabbit polyclonal antibody to ARFGAP1Immunogen:Recombinant full length protein of human ARFGAP1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:ARFGAP1Alternative Names:ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein
Entrez Gene (Human):
55738;
Entrez Gene (Mouse):
228998;
Entrez Gene (Rat):
246310;
SwissProt (Human):
Q8N6T3;
SwissProt (Mouse):
Q9EPJ9;
SwissProt (Rat):
Q62848;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ARFGAP1 expression in Hela (A), HepG2 (B), mouse liver (C), mouse brain (D), rat lung (E) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 45 kD)
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Immunohistochemical analysis of ARFGAP1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.