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CQA1819
  • Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates. (Predicted band size: 84; 132; 135; 139; 142 kD; Observed band size: 143 kD)
  • Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-AMBRA1 Antibody
Cat No:CQA1819
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to AMBRA1
Immunogen:Recombinant full length protein of human AMBRA1
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:AMBRA1
Alternative Names:KIAA1736; Activating molecule in BECN1-regulated autophagy protein 1
Entrez Gene (Human): 55626;
Entrez Gene (Mouse): 228361;
SwissProt (Human): Q9C0C7;
SwissProt (Mouse): A2AH22;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates. (Predicted band size: 84; 132; 135; 139; 142 kD; Observed band size: 143 kD)
  • Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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