Description:Rabbit polyclonal antibody to ACAS2Immunogen:Recombinant full length protein of human ACAS2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:ACSS2Alternative Names:ACAS2; Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme
Entrez Gene (Human):
55902;
Entrez Gene (Mouse):
60525;
SwissProt (Human):
Q9NR19;
SwissProt (Mouse):
Q9QXG4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ACAS2 expression in HepG2 (A), SW620 (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 78; 80 kD; Observed band size: 78 kD)
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Immunofluorescent analysis of ACAS2 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.