Description：Rabbit polyclonal antibody to HMGCLImmunogen：Recombinant full length protein of human HMGCLPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol：HMGCLAlternative Names：Hydroxymethylglutaryl-CoA lyase mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase Entrez Gene (Human)： 3155; Entrez Gene (Rat)： 79238; SwissProt (Human)： P35914; SwissProt (Rat)： P97519; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HMGCL expression in SKOV3 (A), MCF7 (B), mouse liver (C), mouse kidney (D) whole cell lysates. (Predicted band size: 20; 26; 34 kD; Observed band size: 30 kD)
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  Immunohistochemical analysis of HMGCL staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.