Description:Rabbit polyclonal antibody to Caprin1Immunogen:Recombinant full length protein of human Caprin1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:CAPRIN1Alternative Names:GPIAP1; GPIP137; M11S1; RNG105; Caprin-1; Cell cycle-associated protein 1; Cytoplasmic activation- and proliferation-associated protein 1; GPI-anchored membrane protein 1; GPI-anchored protein p137; GPI-p137; p137GPI; Membrane component chromosome 11 surface marker 1; RNA granule protein 105
Entrez Gene (Human):
4076;
Entrez Gene (Mouse):
53872;
Entrez Gene (Rat):
362173;
SwissProt (Human):
Q14444;
SwissProt (Mouse):
Q60865;
SwissProt (Rat):
Q5M9G3;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Caprin1 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 76; 78 kD; Observed band size: 120 kD)
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Immunofluorescent analysis of Caprin1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).