Description：Rabbit polyclonal antibody to Brn-5Immunogen：Recombinant full length protein of human Brn-5Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol：POU6F1Alternative Names：BRN5; MPOU; TCFB1; POU domain, class 6, transcription factor 1; Brain-specific homeobox/POU domain protein 5; Brain-5; Brn-5; mPOU homeobox protein Entrez Gene (Human)： 5463; Entrez Gene (Mouse)： 19009; Entrez Gene (Rat)： 116545; SwissProt (Human)： Q14863; SwissProt (Mouse)： Q07916; SwissProt (Rat)： P56223; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Brn-5 expression in HepG2 (A), Raji (B), mouse liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 45 kD)
• 
  Immunofluorescent analysis of Brn-5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.