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CPA2470
  • Western blot analysis of AKR1A1 expression in rat kidney (A), rat lung (B), mouse kidney (C), mouse lung (D), U2OS (E), Hela (F), HEK293T (G) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
  • Immunohistochemical analysis of AKR1A1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-AKR1A1 Antibody
Cat No:CPA2470
Source:Rabbit
Reactivity:H, M, R, B, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
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200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to AKR1A1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKR1A1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:AKR1A1
Alternative Names:ALDR1; ALR; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Aldo-keto reductase family 1 member A1
Entrez Gene (Human): 10327;
Entrez Gene (Mouse): 58810;
Entrez Gene (Rat): 78959;
SwissProt (Human): P14550;
SwissProt (Mouse): Q9JII6;
SwissProt (Rat): P51635;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of AKR1A1 expression in rat kidney (A), rat lung (B), mouse kidney (C), mouse lung (D), U2OS (E), Hela (F), HEK293T (G) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
  • Immunohistochemical analysis of AKR1A1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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