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CPA2308
  • Western blot analysis of B4GALT3 expression in Jurkat (A), HL60 (B), HeLa (C), COLO205 (D) whole cell lysates. (Predicted band size: 15; 43 kD; Observed band size: 44 kD)
  • Immunohistochemical analysis of B4GALT3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-B4GALT3 Antibody
Cat No:CPA2308
Source:Rabbit
Reactivity:H, M, R, B
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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350
100 μl
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30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to B4GALT3
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human B4GALT3. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:B4GALT3
Alternative Names:Beta-1,4-galactosyltransferase 3; Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Entrez Gene (Human): 8703;
Entrez Gene (Mouse): 57370;
Entrez Gene (Rat): 494342;
SwissProt (Human): O60512;
SwissProt (Mouse): Q91YY2;
SwissProt (Rat): Q6P768;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of B4GALT3 expression in Jurkat (A), HL60 (B), HeLa (C), COLO205 (D) whole cell lysates. (Predicted band size: 15; 43 kD; Observed band size: 44 kD)
  • Immunohistochemical analysis of B4GALT3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1
Journal Cell Death Dis
IF 9
Application WB
Reactivity Human
PMID 23744354
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