Description:Rabbit polyclonal antibody to UbImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ub. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:UBA52; RPS27A; UBB; UBCAlternative Names:UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1
Entrez Gene (Human):
7311;
Entrez Gene (Mouse):
22187;
22190;
78294;
22186;
Entrez Gene (Rat):
192255;
50522;
100912032;
64156;
SwissProt (Human):
P0CG47;
P0CG48;
P62979;
P62987;
SwissProt (Mouse):
P0CG49;
P0CG50;
P62983;
P62984;
SwissProt (Rat):
P0CG51;
Q63429;
P62982;
P62986;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ub expression in mouse liver (A), mouse testis (B), rat liver (C), rat testis (D) whole cell lysates. (Predicted band size: 25; 77; 17; 14 kD; Observed band size: 9 kD)
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Immunohistochemical analysis of Ub staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.