Description：Rabbit polyclonal antibody to TRAF3Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：TRAF3Alternative Names：CAP1; CRAF1; TNF receptor-associated factor 3; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1 Entrez Gene (Human)： 7187; Entrez Gene (Mouse)： 22031; SwissProt (Human)： Q13114; SwissProt (Mouse)： Q60803; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRAF3 expression in MCF7 (A), HeLa (B), Raji (C), NIH3T3 (D), mouse lung (E), rat colon (F), PC12 (G) whole cell lysates. (Predicted band size: 55; 64 kD; Observed band size: 70 kD)
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  Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).