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CPA2183
  • Western blot analysis of Topoisomerase 2 beta expression in HEK293T (A), mouse testis (B) whole cell lysates. (Predicted band size: 183 kD; Observed band size: 183 kD)
  • Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Topoisomerase 2 beta Antibody
Cat No:CPA2183
Source:Rabbit
Reactivity:H, M, D, Mk, P
Applications:WB, IH, ChIP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to Topoisomerase 2 beta
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 beta. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100), ChIP (1/10 - 1/100)
Gene Symbol:TOP2B
Alternative Names:DNA topoisomerase 2-beta; DNA topoisomerase II, beta isozyme
Entrez Gene (Human): 7155;
SwissProt (Human): Q02880;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Topoisomerase 2 beta expression in HEK293T (A), mouse testis (B) whole cell lysates. (Predicted band size: 183 kD; Observed band size: 183 kD)
  • Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
TRF2 and Apollo Cooperate with Topoisomerase 2alpha to Protect Human Telomeres from Replicative Damage
Journal Cell
IF 64.5
Application ChIP
Reactivity Human
PMID 20655466
DNA damage response (DDR) induced by topoisomerase II poisons requires nuclear function of the small GTPase Rac
Journal Biochim Biophys Acta
IF 3
Application
Reactivity
PMID 23999236
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