Description:Rabbit polyclonal antibody to Topoisomerase 2 betaImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 beta. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100), ChIP (1/10 - 1/100)Gene Symbol:TOP2BAlternative Names:DNA topoisomerase 2-beta; DNA topoisomerase II, beta isozyme
Entrez Gene (Human):
7155;
SwissProt (Human):
Q02880;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Topoisomerase 2 beta expression in HEK293T (A), mouse testis (B) whole cell lysates. (Predicted band size: 183 kD; Observed band size: 183 kD)
-
Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
TRF2 and Apollo Cooperate with Topoisomerase 2alpha to Protect Human Telomeres from Replicative Damage
DNA damage response (DDR) induced by topoisomerase II poisons requires nuclear function of the small GTPase Rac