Description：Rabbit polyclonal antibody to Progesterone Receptor (Phospho-S294)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S294 of human Progesterone Receptor protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：PGRAlternative Names：NR3C3; Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3 Entrez Gene (Human)： 5241; Entrez Gene (Rat)： 25154; SwissProt (Human)： P06401; SwissProt (Mouse)： Q00175; SwissProt (Rat)： Q63449; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Progesterone Receptor (Phospho-S294) expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 98 kD; Observed band size: 118 kD)
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  Immunofluorescent analysis of Progesterone Receptor (Phospho-S294) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.