Description:Rabbit polyclonal antibody to Cytochrome P450 1A1/2Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 1A1/2. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:CYP1A1; CYP1A2Alternative Names:CYP1A1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A2; Cytochrome P450 1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3
Entrez Gene (Human):
1543;
1544;
Entrez Gene (Mouse):
13076;
13077;
Entrez Gene (Rat):
24296;
24297;
SwissProt (Human):
P04798;
P05177;
SwissProt (Mouse):
P00184;
P00186;
SwissProt (Rat):
P00185;
P04799;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates. (Predicted band size: 18; 20; 58 kD; Observed band size: 58 kD)
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Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Combination of Coptis chinensis polysaccharides and berberine ameliorates ulcerative colitis by regulating gut microbiota and activating AhR/IL-22 pathway