Description:Rabbit polyclonal antibody to Galectin 1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:LGALS1Alternative Names:Galectin-1; Gal-1; 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1
Entrez Gene (Human):
3956;
Entrez Gene (Mouse):
16852;
Entrez Gene (Rat):
56646;
SwissProt (Human):
P09382;
SwissProt (Mouse):
P16045;
SwissProt (Rat):
P11762;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Galectin 1 expression in A549 (A), HL60 (B) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 15 kD)
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Immunohistochemical analysis of Galectin 1 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.