Description：Recombinant rabbit monoclonal antibody to PSD95Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human PSD95. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：DLG4Alternative Names：PSD95; Disks large homolog 4; Postsynaptic density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90 Entrez Gene (Human)： 1742; Entrez Gene (Mouse)： 13385; Entrez Gene (Rat)： 29495; SwissProt (Human)： P78352; SwissProt (Mouse)： Q62108; SwissProt (Rat)： P31016; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of PSD95 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 90 kD)
• 
  Immunofluorescent analysis of PSD95 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).