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CCP1135
Product Name:Calcineurin Substrate
Cat No:CCP1135
Source:Synthetic
Reactivity:
Applications:
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
1 mg
106
5 mg
318
10 mg
530
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Description:Peptide to Calcineurin SubstRate
Form:Lyophilized powder
CAS Number:113873-67-9
Molecular Formula:C92H150N28O29
Molecular Weight:2112.4
Purity:> 95%
Chemical Structure:Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
Application:The 19-peptide DLDVPIPGRFDRRVSVAAE is a fragment of the RII subunit of the cAMP-dependent protein kinase. It is successfully used in a nonradioactive assay in which both phosphorylation by protein kinase and dephosphorylation by protein phosphatase 2B (calcineurin) are monitored by the simultaneous quantitative determination of phosphorylated and non-phosphorylated peptide using HPLC on an RP18 column with UV detection. This method allows the measurement of enzyme kinetics as well as the characterization of novel inhibitors.
Storage/Stability:Shipped at 4°C. Store at -20°C for one year.
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