Description:Peptide to Calcineurin SubstRateForm:Lyophilized powder
CAS Number:113873-67-9Molecular Formula:C92H150N28O29Molecular Weight:2112.4Purity:> 95%Chemical Structure:Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-GluApplication:The 19-peptide DLDVPIPGRFDRRVSVAAE is a fragment of the RII subunit of the cAMP-dependent protein kinase. It is successfully used in a nonradioactive assay in which both phosphorylation by protein kinase and dephosphorylation by protein phosphatase 2B (calcineurin) are monitored by the simultaneous quantitative determination of phosphorylated and non-phosphorylated peptide using HPLC on an RP18 column with UV detection. This method allows the measurement of enzyme kinetics as well as the characterization of novel inhibitors.Storage/Stability:Shipped at 4°C. Store at -20°C for one year.