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MAP2 Blocking Peptide CBP5291
  • Western blot analysis of MAP2 expression in SKNSH (A), mouse brain (B) whole cell lysates.
  • Immunohistochemical analysis of MAP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameMAP2 Blocking Peptide
Cat No: CBP5291
Source: Synthetic
Reactivity: H, M, R
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-MAP2 Antibody (#CPA5291) reactivity.
Form: Lyophilized powder
Gene Symbol: MAP2
Alternative Names: Microtubule-associated protein 2; MAP-2
Entrez Gene (Human): 4133
Entrez Gene (Mouse) : 17756
Entrez Gene (Rat) : 25595
SwissProt (Human): P11137
SwissProt (Mouse) : P20357
SwissProt (Rat) : P15146
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of MAP2 expression in SKNSH (A), mouse brain (B) whole cell lysates.
  • Immunohistochemical analysis of MAP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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