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SESN1 Blocking Peptide CBP5233
  • Western blot analysis of SESN1 expression in K562 (A), Hela (B), rat muscle (C) whole cell lysates.
  • Immunohistochemical analysis of SESN1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameSESN1 Blocking Peptide
Cat No: CBP5233
Source: Synthetic
Reactivity: H, M, R
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-SESN1 Antibody (#CPA5233) reactivity.
Form: Lyophilized powder
Gene Symbol: SESN1
Alternative Names: PA26; SEST1; Sestrin-1; p53-regulated protein PA26
Entrez Gene (Human): 27244
Entrez Gene (Mouse) : 140742
SwissProt (Human): Q9Y6P5
SwissProt (Mouse) : P58006
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of SESN1 expression in K562 (A), Hela (B), rat muscle (C) whole cell lysates.
  • Immunohistochemical analysis of SESN1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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