Description: The peptide is used to block Anti-POLR3B Antibody (#CPA5206) reactivity. Form: Lyophilized powder Gene Symbol: POLR3B Alternative Names: DNA-directed RNA polymerase III subunit RPC2; RNA polymerase III subunit C2; C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit BEntrez Gene (Human): 55703Entrez Gene (Mouse) : 70428SwissProt (Human): Q9NW08SwissProt (Mouse) : P59470Purity : >85%Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.Storage/Stability : Shipped at 4°C. Store at -20°C for one year.

• 
  Western blot analysis of POLR3B expression in Hela (A), HepG2 (B) whole cell lysates.
• 
  Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.