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ASF1B Blocking Peptide CBP5172
  • Western blot analysis of ASF1B expression in Jurkat (A), Hela (B) whole cell lysates.
  • Immunohistochemical analysis of ASF1B staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameASF1B Blocking Peptide
Cat No: CBP5172
Source: Synthetic
Reactivity: H
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-ASF1B Antibody (#CPA5172) reactivity.
Form: Lyophilized powder
Gene Symbol: ASF1B
Alternative Names: Histone chaperone ASF1B; Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II
Entrez Gene (Human): 55723
SwissProt (Human): Q9NVP2
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of ASF1B expression in Jurkat (A), Hela (B) whole cell lysates.
  • Immunohistochemical analysis of ASF1B staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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