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SUPT16H Blocking Peptide CBP5165
  • Western blot analysis of SUPT16H expression in Hela (A), A549 (B), Jurkat (C) whole cell lysates.
  • Immunohistochemical analysis of SUPT16H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameSUPT16H Blocking Peptide
Cat No: CBP5165
Source: Synthetic
Reactivity: H, M, R
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-SUPT16H Antibody (#CPA5165) reactivity.
Form: Lyophilized powder
Gene Symbol: SUPT16H
Alternative Names: FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16
Entrez Gene (Human): 11198
Entrez Gene (Mouse) : 114741
SwissProt (Human): Q9Y5B9
SwissProt (Mouse) : Q920B9
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of SUPT16H expression in Hela (A), A549 (B), Jurkat (C) whole cell lysates.
  • Immunohistochemical analysis of SUPT16H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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