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TEF Blocking Peptide CBP5135
  • Western blot analysis of TEF expression in Jurkat (A), mouse liver (B) whole cell lysates.
  • Immunohistochemical analysis of TEF staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameTEF Blocking Peptide
Cat No: CBP5135
Source: Synthetic
Reactivity: H, M, R, C
Applications: BL
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
1 mg
$100
5 mg
$300
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Description: The peptide is used to block Anti-TEF Antibody (#CPA5135) reactivity.
Form: Lyophilized powder
Gene Symbol: TEF
Alternative Names: KIAA1655; Thyrotroph embryonic factor
Entrez Gene (Human): 7008
Entrez Gene (Mouse) : 21685
Entrez Gene (Rat) : 29362
SwissProt (Human): Q10587
SwissProt (Mouse) : Q9JLC6
SwissProt (Rat) : P41224
Purity : >85%
Directions for Use : Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours.
Quality Control : The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year.
  • Western blot analysis of TEF expression in Jurkat (A), mouse liver (B) whole cell lysates.
  • Immunohistochemical analysis of TEF staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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